例句與造句

1. Major uses of type II restriction enzymes include gene analysis and cloning.
2. Since their discovery in the early 1970s, type II restriction enzymes have revolutionized the way scientists work with DNA, particularly in genetic engineering and molecular biology.
3. Unlike standard Type II restriction enzymes like EcoRI and BamHI, these enzymes cut DNA outside of their recognition sites and therefore can create non-palindromic overhangs.
4. Additionally, because the final product does not have a Type II restriction enzyme recognition site, the correctly-ligated product cannot be cut again by the restriction enzyme, meaning the reaction is essentially irreversible.
5. In molecular biology, " "'Xho " I "'is a type II restriction enzyme EC that recognise the double-stranded DNA sequence CTCGAG and cleaves after C-1.
6. It's difficult to find type ii restriction enzyme in a sentence. 用type ii restriction enzyme造句挺難的
7. Being a type II restriction enzyme, " Bgl " II does not require ATP ( adenosine triphosphate ) for its enzymatic function, but only requires association with a divalent metal cation, most likely Mg 2 +.
8. "' SmaI "'is a type II restriction enzyme derived from " Serratia marcescens "; it is a neoschizomer of XmaI . SmaI produces blunt-ended fragments whereas XmaI produces a 5?overhang.
9. Unlike type I restriction enzymes, type II restriction endonucleases perform very specific cleaving of DNA . Type I restriction enzymes recognize specific sequences, but cleave DNA randomly at sites other than their recognition site whereas type II restriction enzymes cleave only at their specific recognition site.
10. In 1970, Smith and Kent W . Wilcox discovered the first type II restriction enzyme, that is now called as HindII . Smith went on to discover DNA methylases that constitute the other half of the bacterial host restriction and modification systems, as hypothesized by Werner Arber of Switzerland.
11. Like most type II restriction enzymes, " Bgl " II consists of two identical subunits that form a homopalindromic nucleotide sequence AGATCT, cleaving the scissile phosphodiester bond between the first Adenine and Guanine nucleotides on both strands of the DNA molecule, creating sticky ends with 5'end overhangs.
12. Unlike other restriction enzymes of its class, " Bgl " II has been show to possess some unique structural characteristics, such as a ?-sandwich subdomain, and appears to undergo a unique conformational change upon dimerization, but its overall structure and mechanism of catalysis remain consistent with other type II restriction enzymes.